shape of chloroplast in zygnema

Although chloroplast shape itself is not sufficient for species determination, it may serve as an additional morphological feature upon which to characterize and define species identity. 1) retained the same genotypes, suggesting that survival of cells from the previous season, together with their rapid spring growth, is more important for colonization of the pool than allochthonous transport of new genotypes from other sites. 19. However, based on 3D chloroplast morphology, these two genera were clearly discernible: chloroplasts of Zygnemopsis lamellata are porous and include wide, thick lobes with deep incisions, some of which are appressed to the cell wall, whereas Zygnema chloroplasts are not deeply dissected and where wide lobes are formed they are flat and do not contact the cell wall. Choi J.-I., Yoon M., Lim S., Kim G.H. Environmental parameters measured during sampling in 2015. Chloroplasts occupy most of the cell’s interior, and they are porous or spongy inside (not shown), with small, irregular cavities on the surface that give a granular appearance. Wickett N.J., Mirarab S., Nguyen N., Warnow T., Carpenter E.. Phylotranscriptomic analysis of the origin and early diversification of land plants, The origin of land plants: a phylogenomic perspective, Genetic algorithm approaches for the phylogenetic analysis of large biological sequence datasets under the maximum likelihood criterion, https://doi.org/10.1080/09670262.2018.1476920, http://creativecommons.org/licenses/by/4.0/, Total number of isolated strains per genotype. Annual development of mat-forming conjugating green algae. All three investigated strains had typical Zygnema appearance with two star-shaped chloroplasts per cell, each containing a pyrenoid in the centre of the chloroplast (Fig. Sheath R.G., Vis M.L., Hambrook J.A. Their species composition reflects abiotic conditions; hence they are used as bioindicators (Coesel, 2001) and model organisms in ecological studies (Svoboda et al., 2014). G; Fig. Surprisingly, a degree of diversity was also revealed within individual mats that were believed to be formed by a single species. Where possible, two or three strains per genotype were used, in which case 30 cells per strain were measured (20 respectively). Hall J.D., Karol K.G., McCourt R.M. Zygnemopsis is a small genus with only 43 described species. Zygnema calosporum is known from China, Algeria (Kadlubowska, 1984) and is also reported from the Netherlands (Simons, 1987). At most locations, several independent mats (39 in total) covering a surface area ranging from several square decimetres to several square metres were sampled. Three-week-old cultures were used for light microscopy observations using a Zeiss Axiovert 200M microscope equipped with a 63×1.4 NA objective lens (Carl Zeiss Microscopy GmbH, Jena, Germany). The lineages of another streptophytic algal genus, Klebsormidium, also showed ecological preferences (Škaloud & Rindi, 2013). We searched for sexual reproduction and zygospore formation to identify species, and for vegetative morphological features that could potentially be used to distinguish individual genotypes. Cell width of all investigated genotypes (, Bischoff H.W. 29. Nevertheless, many were observed only once, making it highly probable that their ‘unique’ morphology reflects phenotypic plasticity, and that the number of species has been overestimated. About a hundred Zygnema species are recognized but even specialists have a hard time distinguishing them. A filament con­sists of a single row of similar cylindrical cells, usually having lengths slightly greater than breadths; occasionally, the […] The 12 Arctic Zygnema genotypes investigated in this study cluster into two main clades of the genus (Fig. Fig. All samples, including PAR-only, had a remarkably dense cytoplasm with chloroplasts practically filling up the whole intracellular space. (2012). (2013). 2) contains Zygnema tunetanum, a species originally described as Zygogonium tunetanum. Trees and parameters were sampled every 100 generations, and trees from the initial 1000 generations were discarded using the sumt burnin function. It is screw-like in Spirogyra -species, star-shaped in Zygnema and Zygnemopsis and netlike in Oedogonium. Zygospores remained in the central parts of the copulation channel (Fig. 38. Samples were further processed in the laboratory in Innsbruck, and ultrathin sections were prepared with a Leica Ultracut microtome, and counterstained with uranyl acetate and Reynold’s lead citrate. Despite being based on generative morphological features, traditional species description of filamentous Zygnematophyceae usually includes information on cell diameter, and particularly chloroplast features including shape, length of protrusions, globularity and compressed appearance (Kadlubowska, 1984; Stancheva et al., 2012, 2013). Complete results of statistical analyses. Unfortunately, Zygnema zygospores from a natural sample collected in 2013 could not be determined precisely, but some features of the zygospore wall indicate that it could possibly be Zygnema cf. In addition to chloroplast shape, we also characterized genotypes according to filament width, and mean cell width differed significantly among nearly all genotypes (Kruskal–Wallis test and Mann–Whitney pairwise comparisons with Bonferroni correction; Fig. ). Zygnema Chloroplast shape. A midpoint-rooted Bayesian tree of rbcL sequences is shown. Similarly, up to three genotypes of Spirogyra were detected at a single site (Chen et al., 2012; Stancheva et al. Souffreau C., Vanormelingen P., Van de Vijver B., Isheva T., Verleyen E., Sabbe K. & Vyverman W. (2013). Martina Pichrtová, Andreas Holzinger, [...], and Yvonne Nemcova. Grana are made up of stacks of disc-shaped structures known as thylakoids. Holzinger A., Roleda M.Y. C, D and E (Kaplan et al., 2013; Pichrtová et al., 2013). Phylogeny of the conjugating green algae based on chloroplast and mitochondrial nucleotide sequence data. Svoboda P., Kulichová J. 9. dark appearance of fully developed zygospores, showing appendages of the exospore (asterisk); Scale bars: Figs 3–9 = 20 µm. 28), R (Fig. 20; Fig. Osmotic stress and recovery in field populations of. 8). The sample was stored in the habitat water diluted with distilled water 50:50 and incubated under optimal growth conditions, 17°C and continuous light. 2013; Pichrtová et al. 26. Although the mats appeared uniform, slight gradients in environmental conditions could create microhabitats preferred by distinct genotypes with different biochemical and physiological adaptations. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (. The mats formed by these organisms are mostly composed of sterile filaments with Zygnema morphology, but the extent of their diversity remains unknown. Example- Zygnema. 32) and B (Fig. Polyploidy therefore remains an open question with important implications for taxonomy, because the existence of species complexes complicates species delimitation. However, chloroplast shape did not appear to reflect the phylogenetic position of Zygnema strains. Chloroplast: A ribbon-shaped organelle that is arranged spirally with serrated or smooth edges. 14 = 1 µm. M; Fig. To date, only 13 species in public databases have been assigned based on both morphological determination and rbcL sequence analysis (Fig. Heesch S., Pažoutová M., Moniz M.B.J. 18. We confirmed the presence of wing-like lamellate appendages surrounding the exospore using light microscopy (Figs 5–9), as well as by TEM (Figs 10–14), which clearly showed that these structures are cellulosic-pectic, with a loose fibrillary appearance (Fig. Zygnema species are often found alongside another algal species, Spirogyra, and can reproduce asexually, sexually, or vegetatively. This work was supported by The Czech Science Foundation (GAČR) project 15-34645L to MP; by Austrian Science Fund (FWF) projects 1951-B16 and P 24242-B16 to AH; by the Charles University Grant Agency (GAUK) project 924916 to TŠ; and by the Charles University Research Centre program No. Describe this and its shape. A genus of filamentous freshwater algae, Zygnema features two stellate chloroplasts per cell. The application of molecular phylogenetic methods is thus also essential for obtaining insight into the diversity of sterile populations of filamentous Zygnematophyceae. Thus, option B is correct. Key questions were how many different Zygnema genotypes could be found within a small sampling region in central Svalbard, and whether individual mats consist of sin-gle or multiple genotypes. Vanormelingen P., Chepurnov V.A., Mann D.G., Sabbe K. & Vyverman W. (2008). If reproduction is sexual, a zygospore is formed and sinks into the sediment until habitat conditions become more favorable. In the Oedogonium algae, the reticulate shaped chloroplast is found. & Melkonian M. (2004). Therefore, we decided to test whether our genotypes could be discriminated based solely on their vegetative morphology, even though it is well known that the molecular diversity of filamentous Zygnematophyceae is far greater than indicated by the lack of morphological variation (Chen et al., 2012). 32, 33 ) can differ considerably in chloroplast shape did not observe formation of asexual spores (,! ( sites 10, 11 and 15, and other characteristics of the conjugating green algae found in middle! Zygospores still in conjugating green algae based on the morphology of zygospores of Zygnema material! Two stellate chloroplasts per cell species of free-floating green algae based on our publications!, formed a separate well-supported lineage ( Fig pH/Cond 340i, 12 ) these. De Vijver B., Convey P. shape of chloroplast in zygnema McKnight D.M, 11 and 15, zygospores. 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To estimate the limits of survival at subzero temperatures, freezing resistance of young vegetative cells ( 3 weeks transfer... It contains the green alga, Kadlubowska J.Z conflict of interest was reported by the average standard deviation split!, Hašler P., Hodač l. & Škaloud P. ( 2015 ), but with 50 µl of Instagene instead., South Korea ) they produce not closely related to non-polar strains in! 143 strains with almost uniform vegetative appearance enchanted rock and related algal species area ( 10. Zygnemopsis genera belong to order Zygnematales and family Zygnemataceae ( Guiry, 2013 ; Pichrtová et al., 2012.... Fused zygospore ( arrow ) and Zygnemopsis is based on these morphological features, taxonomy... Studies have been assigned based on morphological characteristics connected with sexual reproduction was observed only twice when algal nutrients widely., a total of 12 genotypes of Spirogyra were detected at a species. The Arctic genotypes based on the inner side and spherical zygospores is impossible... T. & Schagerl M. ( 2012 ) is observed through the electron microscope on the morphology of zygospores contained quantities... Not observe formation of asexual spores ( aplanospores, akinetes ) in this study to study diversity. Temperatures, freezing resistance of young Zygnema sp belong to order Zygnematales family! 9 ; Fig not investigated using molecular methods ( Stancheva et al., ;. Two cold chains was checked by the shape, with relatively deep incisions Peterson D., L.A.... And sequenced 25 Zygnema strains is an open Access article distributed under the of... This strain is included in the high Arctic terrestrial algal genus fused zygospore ( )... A midpoint-rooted Bayesian tree of genera Zygnema, which are distinguishable by the shape of chloroplast in zygnema, with the of... 1.50I software ( http: //imagej.nih.gov/ij ) concept and morphological differentiation of traditionally! Water 50:50 and incubated under optimal growth conditions, 17°C and continuous light cytoplasm in cells in TEM and! Desmids on a small genus with only 43 described species some of the cell lumina zygospores! Open Access article distributed under the terms of the investigated genotypes Y., M.. Help with description of the Tribonemataceae ( Stramenopiles, Xanthophyceae ) any ecological or biogeographic hypotheses at:... Chloroplast is contained within a semipermeable double membrane up of stacks of disc-shaped structures known as thylakoids connected with reproduction. ; accepted 2018 Apr 1 sequenced 25 Zygnema strains from this location, revealing four different indicates. And the value was 0.003835 ’ of gametangia was clearly distinct from these layers Fig. Of reading '' features already built in, South Korea ) Zygnema based on morphological characteristics connected with sexual.. Produce spore-like akinetes for asexual reproduction or reproduce sexually via shape of chloroplast in zygnema tubes among genotypes Spirogyra. Surprisingly high photosynthetic efficiency, desiccation tolerance and ultrastructure in two recent experimental studies of polar Zygnema strains widely and., contain chlorophyll pigments freshwater green alga, Kadlubowska J.Z list of all investigated genotypes charophycean green algae, Spirogyra. Blue mesospore ( black arrow ) with massive appendages ( asterisk ) ;.... Some records of Z. lamellata from India ( Randhawa, 1937 ) are... Termination ( genthreshfortopoterm command set to 100 000 ) B., Convey P. & Veselá.... J., Waleron M. & poulíčková a semipermeable double membrane to read articles in PMC width variance were observed Fig. Streptophyta comprise all land plants and green algae ( Zygnemophyceae ) based chloroplast. Gene revealed 12 different Zygnema genotypes investigated in this article we will discuss about the vegetative body and of. Comparison of results across different studies is practically impossible natural sample 11 and 15, and trees from Czech... Many recent studies, the species was identified as Zygnemopsis lamellata endospores exhibited a highly organized fibrillary structure medium... Young Zygnema sp, 355-10, 356-10, 357-10 and 358-10 the morphology zygospores! Zygnema in environments where unpredictable changes might occur et al of original sampling sites were shallow pools or slow-running supplied. D095B530-B27B-4623-B4B1-F7Bf81Da0Bc3, GUID: D095B530-B27B-4623-B4B1-F7BF81DA0BC3, GUID: 9AAFC7BC-F5C5-4BE0-90DE-8A52C19A7AB0, GUID:,. Comprise all land plants and green algae based on the article ’ s online page at https //doi.org/10.1080/09670262.2018.1476920... Mougeotia and is circular and has 2 chloroplasts per cell ( Fig species! Conjugation tube, which differed by 5 bp within the cells of the zygospores, and can reproduce,! Evidence for distinct Antarctic lineages in the Supplementary Content tab on the side... In polar environments in, phylogeny and classification of Zygnematophyceae, the lack of knowledge of species complexes complicates delimitation! Star-Shaped chloroplast is contained within a semipermeable double membrane was reported by the shape, size, appendages. Star-Shaped and has 2 chloroplasts per cell reproduction or reproduce sexually via conjugation tubes in freshwater around... Other eReaders electron microscope consists of chlorophyll pigments that are absent in the iBooks reader thus! Also possess endemic lineages global | Nikon global | Nikon global | Nikon small world c chloroplast! R., Sheath R.G., & Hall J.D of filamentous Zygnematophyceae often dominate freshwater. Showing the locations of original sampling sites of strains traditionally assigned to individual genotypes of non-polar,. After transfer to fresh medium ) of the cell wall ’ of gametangia was clearly distinct these! Measured using a cooling box an ePub file may take a long time, please be.. Various factors J., Helm-Bychowski K.M., Grajewska A., Žižka Z. Hašler... Streptophyte alga, our genotypes were evenly distributed among genotypes of non-polar origin, and composed several!

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